Next generation fungicides - translating dsRNA technology from the lab to the field.

Dr. Steve Robinson, AAFC-Saskatoon

Start Date: April 1, 2020                 

End Date: March 31, 2024

AWC’s funding: $82,500

This project will investigate the application of RNA-based fungicides to control major diseases of crops grown on the prairies, namely wheat and canola. This approach is based on proven research that has demonstrated that the application of RNA molecules directly as a spray can target and silence functionally important pathogen genes. Induction of gene silencing kills the pathogen and prevents further disease formation and spread. Additionally, the systemic nature of RNA-based fungicides can reduce the risks associated with timing of fungicide application to control disease. This proposal builds on the innovations made during ADF project #20170241 “Next Generation Fungicides - Protecting Saskatchewan Crops Using a Revolutionary Approach for Controlling Persistent Crop Disease” that led to the discovery of new targets able to limit pathogen growth through dsRNA spray application. These targets have been evaluated under controlled greenhouse conditions where effective pathogen control has been demonstrated. This proposal pursues four major goals: 1) It will evaluate the existing technology in the field, demonstrating the efficacy of the previously identified targets against FHB - translating the technology from the lab into the field; 2) It will continue the discovery and evaluation of new gene targets enabling further optimization of FHB spray formulations; and 3) Expand application of this technology providing protection against Sclerotinia in oilseed crops using homologous gene targets have proven successful to control FHB.